中文Porcine epidemic diarrhea virus RT-PCR detection kit
Khoom npe
Porcine epidemic diarrhea virus RT-PCR Detection Kit (Lyophilized)
Loj
48tests/kit, 50test/kitab
Npaj siv
Cov khoom siv no siv lub sijhawm tiag tiag fluorescent RT-PCR txoj hauv kev los kuaj xyuas RNA ntawm Porcine epidemic diarrhea virus (PEDV) hauv cov ntaub so ntswg kab mob xws li tonsils, lymph nodes thiab spleen thiab kua kab mob cov ntaub ntawv xws li tshuaj tiv thaiv thiab ntshav ntawm npua.Nws yog qhov tsim nyog rau kev tshawb nrhiav, kev kuaj mob thiab kev tshawb nrhiav kev sib kis ntawm Porcine epidemic diarrhea virus .Cov khoom siv yog ALL-READY PCR SYSTEM (Lyophilized), uas muaj qhov rov qab transcriptase, DNA amplification enzyme, cov tshuaj tiv thaiv tsis zoo, cov primers tshwj xeeb thiab kev sojntsuam xav tau rau fluorescent RT-PCR nrhiav..
Cov khoom txheem
| Cheebtsam | Pob | specification | Cov khoom xyaw |
| PEDV PCR Mix | 1 × lub raj mis (Lyophilized hmoov) | 50 Test | dNTPs, MgCl2, Primers, Probes, Taq DNA polymerase |
| 6 × 0.2ml 8 zoo-strip raj(Lyophilized) | 48 Test | ||
| Kev Tswj Zoo | 1 * 0.2ml tube (lyophilized) | 10 Kev Xeem | Plasmid lossis Pseudovirus muaj PEDV cov khoom tawg |
| Kev daws teeb meem | 1.5 ml Cryotube | 500 ua | / |
| Kev tswj tsis zoo | 1.5 ml Cryotube | 200 ua | 0.9% NaCl |
Cia & Txee Lub neej
(1) Cov khoom siv tuaj yeem thauj tau ntawm chav tsev kub.
(2) Lub neej txee yog 18 lub hlis ntawm -20 ℃ thiab 12 lub hlis ntawm 2 ℃ ~ 30 ℃.
(3) Saib daim ntawv lo ntawm cov khoom siv rau hnub tsim khoom thiab hnub tas sijhawm.
(4) Cov lyophilized hmoov version reagent yuav tsum tau khaws cia ntawm -20 ℃ tom qab dissolution thiab rov qab khov -thaw yuav tsum tsawg tshaj li 4 zaug.
Cov cuab yeej
GENECHECKER UF-150, UF-300 real-time fluorescence PCR ntsuas.
Daim duab ua haujlwm
a) Lub raj mis version:
b) 8 zoo-strip raj version:
Pcr Amplification
Pom zoo teeb tsa
| Kauj ruam | Lub voj voog | Kub (℃) | Sijhawm | Fluorescence channel |
| 1 | 1 | 48 | 8 feeb | / |
| 2 | 1 | 95 | 2 min | / |
| 3 | 40 | 95 | 5s | / |
| 60 | 10s ib | Sau FAM fluorescence |
* Ceeb toom: Cov teeb liab ntawm FAM fluorescence channel yuav raug sau ntawm 60 ℃.
Txhais cov qhabnias xeem
| Channel | Kev txhais cov txiaj ntsig |
| FAM Channel | |
| Ct≤35 | PEDV Positive |
| Undet | PEDV Negative |
| 35 | Cov txiaj ntsig tsis txaus ntseeg, rov kuaj dua * |
*Yog hais tias qhov kev ntsuam xyuas ntawm FAM channel muaj tus nqi Ct ≤40 thiab qhia tau hais tias "S" zoo li qhov nthuav dav nkhaus, qhov tshwm sim tau txhais tias qhov zoo, txwv tsis pub nws yog qhov tsis zoo.
