Vibrio parahaemolyticus PCR Detection Kit
Khoom npe
Vibrio Parahaemolyticus PCR Detection Kit (Lyophilized)
Loj
48tests/kit, 50test/kitab
Npaj siv
Vibrio Parahemolyticus (tseem hu ua Halophile Vibrio Parahemolyticus) yog Gram-negative polymorphic bacillus lossis Vibrio Parahemolyticus.nrog rau qhov pib mob, mob plab, ntuav, raws plab thiab cov quav dej raws li cov tsos mob tseem ceeb ntawm cov khoom siv hauv lub sijhawm. PCR thiab tsim nyog rau kev kuaj pom zoo ntawm Vibrio Parahaemolyticus hauv cov zaub mov, kuaj dej, quav, ntuav, thiab ua kom cov kua dej ntxiv.Cov khoom siv yog ALL-READY PCR SYSTEM (Lyophilized), uas muaj cov DNA amplification enzyme, cov tshuaj tiv thaiv tsis zoo, tshwj xeeb primers thiab cov kev sojntsuam xav tau rau kev kuaj pom fluorescent RT-PCR.
Cov khoom txheem
Cheebtsam | Pob | specification | Cov khoom xyaw |
PCR Mix | 1 × lub raj mis (Lyophilized hmoov) | 50 Test | dNTPs, MgCl2, Primers, Probes, Taq DNA polymerase |
6 × 0.2ml 8 zoo-strip raj(Lyophilized) | 48 Test | ||
Kev Tswj Zoo | 1 * 0.2ml tube (lyophilized) | 10 Kev Xeem | Plasmid lossis Pseudovirus muaj cov khoom tawg |
Kev daws teeb meem | 1.5 ml Cryotube | 500 ua | / |
Kev tswj tsis zoo | 1.5 ml Cryotube | 100 ua | 0.9% NaCl |
Cia & Txee Lub neej
(1) Cov khoom siv tuaj yeem thauj tau ntawm chav tsev kub.
(2) Lub neej txee yog 18 lub hlis ntawm -20 ℃ thiab 12 lub hlis ntawm 2 ℃ ~ 30 ℃.
(3) Saib daim ntawv lo ntawm cov khoom siv rau hnub tsim khoom thiab hnub tas sijhawm.
(4) Cov lyophilized hmoov version reagent yuav tsum tau muab cia rau ntawm -20 ℃ tom qab dissolution thiab rov qab khov -thaw yuav tsum tsawg tshaj li 4 zaug.
Cov cuab yeej
GENECHECKER UF-150, UF-300 real-time fluorescence PCR ntsuas.
Daim duab ua haujlwm
a) Lub raj mis version:
b) 8 zoo-strip raj version:
Pcr Amplification
Pom zoo teeb tsa
Kauj ruam | Lub voj voog | Kub (℃) | Sijhawm | Fluorescence channel |
1 | 1 | 95 | 2 min | / |
2 | 40 | 95 | 6s | / |
60 | 12s ib | Sau FAM fluorescence |
Txhais cov qhabnias xeem
Channel | Kev txhais cov txiaj ntsig |
FAM Channel | |
Ct≤35 | Vibrio Parahaemolyticus Positive |
Undet | Vibrio Parahaemolyticus tsis zoo |
35 | Kev tsis txaus ntseeg, rov ntsuas dua * |
*Yog hais tias qhov kev ntsuam xyuas ntawm FAM channel muaj tus nqi Ct ≤40 thiab qhia tau hais tias "S" zoo li qhov nthuav dav nkhaus, qhov tshwm sim tau txhais tias qhov zoo, txwv tsis pub nws yog qhov tsis zoo.