中文Staphylococcus aureus PCR Detection Kit
Khoom npe
Staphylococcus aureus PCR Detection Kit (Lyophilized)
Loj
48tests/kit, 50test/kitab
Npaj siv
Staphylococcus aureus belongs rau genus Staphylococcus thiab yog cov kab mob gram-zoo.Nws yog ib qho khoom noj uas muaj kab mob ua rau cov kab mob uas tuaj yeem tsim cov enterotoxins thiab ua rau zaub mov lom.Cov khoom siv no siv lub hauv paus ntsiab lus ntawm lub sijhawm tiag tiag fluorescent PCR thiab tsim nyog rau kev kuaj pom zoo ntawm Staphylococcus aureus hauv cov zaub mov, dej kuaj, quav, ntuav, ua kua thiab lwm yam qauv.
Cov khoom txheem
| Cheebtsam | Pob | specification | Cov khoom xyaw |
| PCR Mix | 1 × lub raj mis (Lyophilized hmoov) | 50 Test | dNTPs, MgCl2, Primers, Probes, Taq DNA polymerase |
| 6 × 0.2ml 8 zoo-strip raj(Lyophilized) | 48 Test | ||
| Kev Tswj Zoo | 1 * 0.2ml tube (lyophilized) | 10 Kev Xeem | Plasmid lossis Pseudovirus muaj cov khoom tawg |
| Kev daws teeb meem | 1.5 ml Cryotube | 500 ua | / |
| Kev tswj tsis zoo | 1.5 ml Cryotube | 100 ua | 0.9% NaCl |
Cia & Txee Lub neej
(1) Cov khoom siv tuaj yeem thauj tau ntawm chav tsev kub.
(2) Lub neej txee yog 18 lub hlis ntawm -20 ℃ thiab 12 lub hlis ntawm 2 ℃ ~ 30 ℃.
(3) Saib daim ntawv lo ntawm cov khoom siv rau hnub tsim khoom thiab hnub tas sijhawm.
(4) Cov lyophilized hmoov version reagent yuav tsum tau muab cia rau ntawm -20 ℃ tom qab dissolution thiab rov qab khov -thaw yuav tsum tsawg tshaj li 4 zaug.
Cov cuab yeej
GENECHECKER UF-150, UF-300 real-time fluorescence PCR ntsuas.
Daim duab ua haujlwm
a) Lub raj mis version:
b) 8 zoo-strip raj version:
Pcr Amplification
Pom zoo teeb tsa
| Kauj ruam | Lub voj voog | Kub (℃) | Sijhawm | Fluorescence channel |
| 1 | 1 | 95 | 2 min | / |
| 2 | 40 | 95 | 6s | / |
| 60 | 12s ib | Sau FAM fluorescence |
Txhais cov qhabnias xeem
| Channel | Kev txhais cov txiaj ntsig |
| FAMChannel | |
| Ct≤35 | Staphylococcus aureus Qhov zoo |
| Undet | Staphylococcus aureus Tsis zoo |
| 35<Ct≤40 | Muaj kev xav tsis thoobrov qab,rov ntsuam xyuas * |
*Yogtus retest tau ntawm FAM channelhastus nqi Ct ≤40thiab qhia tau hais tias raug“S”duab amplification nkhaus, qhov tshwm sim yog txhais raws li qhov zoo, txwv tsis pub nws yog qhov tsis zoo.
