中文Shigella PCR Detection Kit
Khoom npe
Shigella PCR Detection Kit (Lyophilized)
Loj
48tests/kit, 50test/kitab
Npaj siv
Shigella yog ib hom kab mob gram-negative brevis bacilli, teej tug mus rau cov kab mob plab hnyuv, thiab feem ntau cov kab mob ntawm tib neeg bacillary dysentery.Cov khoom siv no siv lub hauv paus ntsiab lus ntawm lub sijhawm tiag tiag fluorescent PCR, uas tsim nyog rau kev txiav txim siab ntawm Shigella hauv cov zaub mov, cov qauv dej, cov quav, ntuav, thiab cov kua dej ntxiv.
Cov khoom txheem
| Cheebtsam | Pob | specification | Cov khoom xyaw |
| PCR Mix | 1 × lub raj mis (Lyophilized hmoov) | 50 Test | dNTPs, MgCl2, Primers, Probes, Taq DNA polymerase |
| 6 × 0.2ml 8 zoo-strip raj(Lyophilized) | 48 Test | ||
| Kev Tswj Zoo | 1 * 0.2ml tube (lyophilized) | 10 Kev Xeem | Plasmid lossis Pseudovirus muaj cov khoom tawg |
| Kev daws teeb meem | 1.5 ml Cryotube | 500 ua | / |
| Kev tswj tsis zoo | 1.5 ml Cryotube | 100 ua | 0.9% NaCl |
Cia & Txee Lub neej
(1) Cov khoom siv tuaj yeem thauj tau ntawm chav tsev kub.
(2) Lub neej txee yog 18 lub hlis ntawm -20 ℃ thiab 12 lub hlis ntawm 2 ℃ ~ 30 ℃.
(3) Saib daim ntawv lo ntawm cov khoom siv rau hnub tsim khoom thiab hnub tas sijhawm.
(4) Cov lyophilized hmoov version reagent yuav tsum tau muab cia rau ntawm -20 ℃ tom qab dissolution thiab rov qab khov -thaw yuav tsum tsawg tshaj li 4 zaug.
Cov cuab yeej
GENECHECKER UF-150, UF-300 real-time fluorescence PCR ntsuas.
Daim duab ua haujlwm
a) Lub raj mis version:
b) 8 zoo-strip raj version:
Pcr Amplification
Pom zoo teeb tsa
| Kauj ruam | Lub voj voog | Kub (℃) | Sijhawm | Fluorescence channel |
| 1 | 1 | 95 | 2 min | / |
| 2 | 40 | 95 | 5s | / |
| 60 | 10s ib | Sau FAM fluorescence |
Txhais cov qhabnias xeem
| Channel | Kev txhais cov txiaj ntsig |
| FAM Channel | |
| Ct≤35 | Shigella Positive |
| Undet | Shigella Negative |
| 35 | Kev tsis txaus ntseeg, rov ntsuas dua * |
*Yog hais tias qhov kev ntsuam xyuas ntawm FAM channel muaj tus nqi Ct ≤40 thiab qhia tau hais tias "S" zoo li qhov nthuav dav nkhaus, qhov tshwm sim tau txhais tias qhov zoo, txwv tsis pub nws yog qhov tsis zoo.
