中文Salmonella PCR Detection Kit
Khoom npe
Salmonella PCR Detection Kit (Lyophilized)
Loj
48tests/kit, 50test/kitab
Npaj siv
Salmonella belongs rau Enterobacteriaceae thiab Gram-negative enterobacteria.Salmonella yog ib hom kab mob uas kis tau los ntawm cov zaub mov thiab yog thawj zaug hauv cov kab mob zaub mov lom.Cov tsos mob tseem ceeb ntawm kev noj zaub mov lom los ntawm Salmonella yog xeev siab, ntuav, mob plab, mob taub hau, ua daus no thiab raws plab.Cov khoom siv no siv lub hauv paus ntsiab lus ntawm lub sijhawm tiag tiag fluorescent PCR thiab tsim nyog rau kev kuaj pom tus kab mob Salmonella hauv cov zaub mov, kuaj dej, quav, ntuav, thiab cov kua dej ntxiv.
Cov khoom txheem
| Cheebtsam | Pob | specification | Cov khoom xyaw |
| PCR Mix | 1 × lub raj mis (Lyophilized hmoov) | 50 Test | dNTPs, MgCl2, Primers, Probes, Taq DNA polymerase |
| 6 × 0.2ml 8 zoo-strip raj(Lyophilized) | 48 Test | ||
| Kev Tswj Zoo | 1 * 0.2ml tube (lyophilized) | 10 Kev Xeem | Plasmid lossis Pseudovirus muaj cov khoom tawg |
| Kev daws teeb meem | 1.5 ml Cryotube | 500 ua | / |
| Kev tswj tsis zoo | 1.5 ml Cryotube | 100 ua | 0.9% NaCl |
Cia & Txee Lub neej
(1) Cov khoom siv tuaj yeem thauj tau ntawm chav tsev kub.
(2) Lub neej txee yog 18 lub hlis ntawm -20 ℃ thiab 12 lub hlis ntawm 2 ℃ ~ 30 ℃.
(3) Saib daim ntawv lo ntawm cov khoom siv rau hnub tsim khoom thiab hnub tas sijhawm.
(4) Cov lyophilized hmoov version reagent yuav tsum tau muab cia rau ntawm -20 ℃ tom qab dissolution thiab rov qab khov -thaw yuav tsum tsawg tshaj li 4 zaug.
Cov cuab yeej
GENECHECKER UF-150, UF-300 real-time fluorescence PCR ntsuas.
Daim duab ua haujlwm
a) Lub raj mis version:
b) 8 zoo-strip raj version:
Pcr Amplification
Pom zoo teeb tsa
| Kauj ruam | Lub voj voog | Kub (℃) | Sijhawm | Fluorescence channel |
| 1 | 1 | 95 | 2 min | / |
| 2 | 40 | 95 | 5s | / |
| 60 | 10s ib | Sau FAM fluorescence |
Txhais cov qhabnias xeem
| Channel | Kev txhais cov txiaj ntsig |
| FAM Channel | |
| Ct≤35 | Salmonella Positive |
| Undet | Salmonella Negative |
| 35 | Kev tsis txaus ntseeg, rov ntsuas dua * |
*Yog hais tias qhov kev ntsuam xyuas ntawm FAM channel muaj tus nqi Ct ≤40 thiab qhia tau hais tias "S" zoo li qhov nthuav dav nkhaus, qhov tshwm sim tau txhais tias qhov zoo, txwv tsis pub nws yog qhov tsis zoo.
