Pseudorabies virus (gB) PCR kuaj cov khoom siv

Lus piav qhia luv luv:

Cov khoom siv no siv cov txheej txheem fluorescent PCR ntawm lub sijhawm tiag tiag los kuaj xyuas tus kab mob RNA ntawm Pseudorabies virus (gB gene) (PRV) hauv cov ntaub so ntswg kab mob xws li tonsils, lymph nodes thiab spleen thiab cov ntaub ntawv kab mob ua kua xws li tshuaj tiv thaiv thiab ntshav ntawm npua.


Product Detail

Khoom cim npe

Khoom npe

Pseudorabies virus (gB) PCR Detection Kit (Lyophilized)

Loj

48tests/kit, 50test/kitab

Npaj siv

Cov khoom siv no siv cov txheej txheem fluorescent PCR ntawm lub sijhawm tiag tiag los kuaj xyuas tus kab mob RNA ntawm Pseudorabies virus (gB gene) (PRV) hauv cov ntaub so ntswg kab mob xws li tonsils, lymph nodes thiab spleen thiab cov ntaub ntawv kab mob ua kua xws li tshuaj tiv thaiv thiab ntshav ntawm npua.Nws yog qhov tsim nyog rau kev tshawb nrhiav, kev kuaj mob thiab kev tshawb nrhiav kev sib kis ntawm tus kab mob Pseudorabies (gB gene).Cov khoom siv yog ALL-READY PCR SYSTEM (Lyophilized), uas muaj cov DNA amplification enzyme, cov tshuaj tiv thaiv tsis zoo, cov primers tshwj xeeb thiab cov kev sojntsuam xav tau rau fluorescent RT-PCR nrhiav pom.

Cov khoom txheem

Cheebtsam Pob specification Cov khoom xyaw
PRV PCR Mix 1 × lub raj mis (Lyophilized hmoov)  50 Test dNTPs, MgCl2, Primers, Probes, Taq DNA polymerase
6 × 0.2ml 8 zoo-strip raj(Lyophilized) 48 Test
Kev Tswj Zoo 1 * 0.2ml tube (lyophilized)  10 Kev Xeem

Plasmid lossis Pseudovirus muaj PRV (gB) cov khoom tawg

Kev daws teeb meem 1.5 ml Cryotube 500 ua /
Kev tswj tsis zoo 1.5 ml Cryotube 200 ua 0.9% NaCl

Cia & Txee Lub neej

(1) Cov khoom siv tuaj yeem thauj tau ntawm chav tsev kub.

(2) Lub neej txee yog 18 lub hlis ntawm -20 ℃ thiab 12 lub hlis ntawm 2 ℃ ~ 30 ℃.

(3) Saib daim ntawv lo ntawm cov khoom siv rau hnub tsim khoom thiab hnub tas sijhawm.

(4) Cov lyophilized hmoov version reagent yuav tsum tau muab cia rau ntawm -20 ℃ tom qab dissolution thiab rov qab khov -thaw yuav tsum tsawg tshaj li 4 zaug.

Cov cuab yeej

GENECHECKER UF-150, UF-300 real-time fluorescence PCR ntsuas.

Daim duab ua haujlwm

a) Lub raj mis version:

1

b) 8 zoo-strip raj version:

2

Pcr Amplification

Pom zoo teeb tsa

Kauj ruam

Lub voj voog

Kub (℃)

Sijhawm

Fluorescence channel

1

1

95

2 min

/

2

40

95

5s

/

60

10s ib

Sau FAM fluorescence

* Ceeb toom: Cov teeb liab ntawm FAM fluorescence channel yuav raug sau ntawm 60 ℃.

Txhais cov qhabnias xeem

Channel

Kev txhais cov txiaj ntsig

FAM Channel

Ct≤35

PRV(gB) Positive

Undet

PRV(gB) Negative

35

Kev tsis txaus ntseeg, rov ntsuas dua *

*Yog hais tias qhov kev ntsuam xyuas ntawm FAM channel muaj tus nqi Ct ≤40 thiab qhia tau hais tias "S" zoo li qhov nthuav dav nkhaus, qhov tshwm sim tau txhais tias qhov zoo, txwv tsis pub nws yog qhov tsis zoo.


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