中文Norovirus (GⅠ) RT-PCR Detection Kit
Khoom npe
Norovirus (GⅠ) RT-PCR Detection Kit (Lyophilized)
Loj
48tests/kit, 50test/kitab
Npaj siv
Nws yog qhov tsim nyog rau kev kuaj pom tus kab mob Norovirus (GⅠ) hauv plhaub ntses, zaub nyoos thiab txiv hmab txiv ntoo, dej, quav, ntuav thiab lwm yam qauv.Nucleic acid extraction yuav tsum tau nqa tawm los ntawm nucleic acid extraction cov khoom los yog ncaj qha pyrolysis txoj kev raws li cov qauv sib txawv.Cov khoom siv yog ALL-READY PCR SYSTEM (Lyophilized), uas muaj cov DNA amplification enzyme, thim rov qab transcriptase, tshuaj tsis haum, tshwj xeeb primers thiab kev sojntsuam xav tau rau kev kuaj pom fluorescent PCR.
Cov khoom txheem
| Cheebtsam | Pob | specification | Cov khoom xyaw |
| Norovirus (GⅠ) PCR Mix | 1 × lub raj mis (Lyophilized hmoov) | 50 Test | dNTPs, MgCl2, Primers, Probes, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 zoo-strip raj(Lyophilized) | 48 Test | ||
| Kev Tswj Zoo | 1 * 0.2ml tube (lyophilized) | 10 Kev Xeem | Plasmid muaj Norovirus (GⅠ) cov khoom tawg |
| Kev daws teeb meem | 1.5 ml Cryotube | 500 ua | / |
| Kev tswj tsis zoo | 1.5 ml Cryotube | 200 ua | 0.9% NaCl |
Cia & Txee Lub neej
(1) Cov khoom siv tuaj yeem thauj tau ntawm chav tsev kub.
(2) Lub neej txee yog 18 lub hlis ntawm -20 ℃ thiab 12 lub hlis ntawm 2 ℃ ~ 30 ℃.
(3) Saib daim ntawv lo ntawm cov khoom siv rau hnub tsim khoom thiab hnub tas sijhawm.
(4) Cov lyophilized hmoov version reagent yuav tsum tau muab cia rau ntawm -20 ℃ tom qab dissolution thiab rov qab khov -thaw yuav tsum tsawg tshaj li 4 zaug.
Cov cuab yeej
GENECHECKER UF-150, UF-300 real-time fluorescence PCR ntsuas.
Daim duab ua haujlwm
a) Lub raj mis version:
b) 8 zoo-strip raj version:
Pcr Amplification
Pom zooKev teeb tsa
| Kauj ruam | Lub voj voog | Kub (℃) | Sijhawm | Fluorescence channel |
| 1 | 1 | 50 | 8 feeb | |
| 2 | 1 | 95 | 2 min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s ib | Sau FAM fluorescence |
* Ceeb toom: Cov teeb liab ntawm FAM fluorescence channel yuav raug sau ntawm 60 ℃.
Txhais cov qhabnias xeem
| Channel | Kev txhais cov txiaj ntsig |
| FAM Channel | |
| Ct≤35 | Norovirus (GⅠ) Positive |
| Undet | Norovirus (GⅠ) Tsis zoo |
| 35 | Kev tsis txaus ntseeg, rov ntsuas dua * |
*Yog hais tias qhov kev ntsuam xyuas ntawm FAM channel muaj tus nqi Ct ≤40 thiab qhia tau hais tias "S" zoo li qhov nthuav dav nkhaus, qhov tshwm sim tau txhais tias qhov zoo, txwv tsis pub nws yog qhov tsis zoo.
