中文E.coli O157:H7 PCR kuaj cov khoom siv
Khoom npe
E.coli O157: H7 PCR kuaj cov khoom (Lyophilized)
Loj
48tests/kit, 50test/kitab
Npaj siv
Escherichia coli O157:H7 (E.coli O157:H7) yog ib hom kab mob gram-negative uas yog cov genus Enterobacteriaceae, uas ua rau muaj ntau ntawm Vero toxin.Clinically, nws feem ntau tshwm sim dheev nrog mob plab thiab dej raws plab, ua raws li hemorrhagic raws plab ob peb hnub tom qab, uas tuaj yeem ua rau kub taub hau lossis tsis ua npaws, thiab tuag rau qhov mob hnyav.Cov khoom siv no tsim nyog rau kev kuaj pom zoo ntawm Escherichia coli O157: H7 hauv cov khoom noj, cov qauv dej, cov quav, ntuav, cov kab mob ua kom cov kua thiab lwm yam qauv siv cov qauv ntawm lub sijhawm PCR.Cov khoom siv yog ALL-READY PCR SYSTEM ( Lyophilized), uas muaj cov DNA amplification enzyme, cov tshuaj tiv thaiv tsis zoo, tshwj xeeb primers thiab sojntsuam xav tau rau fluorescent PCR nrhiav tau.
Cov khoom txheem
| Cheebtsam | Pob | specification | Cov khoom xyaw |
| E.coli O157:H7 PCR Mix | 1 × lub raj mis (Lyophilized hmoov) | 50 Test | dNTPs, MgCl2, Primers, Probes, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 zoo-strip raj(Lyophilized) | 48 Test | ||
| Kev Tswj Zoo | 1 * 0.2ml tube (lyophilized) | 10 Kev Xeem | Plasmid muaj E.coli O157: H7 cov khoom tawg |
| Kev daws teeb meem | 1.5 ml Cryotube | 500 ua | / |
| Kev tswj tsis zoo | 1.5 ml Cryotube | 200 ua | 0.9% NaCl |
Cia & Txee Lub neej
(1) Cov khoom siv tuaj yeem thauj tau ntawm chav tsev kub.
(2) Lub neej txee yog 18 lub hlis ntawm -20 ℃ thiab 12 lub hlis ntawm 2 ℃ ~ 30 ℃.
(3) Saib daim ntawv lo ntawm cov khoom siv rau hnub tsim khoom thiab hnub tas sijhawm.
(4) Cov lyophilized hmoov version reagent yuav tsum tau muab cia rau ntawm -20 ℃ tom qab dissolution thiab rov qab khov -thaw yuav tsum tsawg tshaj li 4 zaug.
Cov cuab yeej
GENECHECKER UF-150, UF-300 real-time fluorescence PCR ntsuas.
Daim duab ua haujlwm
a) Lub raj mis version:
b) 8 zoo-strip raj version:
Pcr Amplification
Pom zooKev teeb tsa
| Kauj ruam | Lub voj voog | Kub (℃) | Sijhawm | Fluorescence channel |
| 1 | 1 | 95 | 2 min | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s ib | Sau FAM fluorescence |
* Ceeb toom: Lub teeb liab ntawm FAM fluorescence channel yuav raug sau ntawm 60 ℃.
Txhais cov qhabnias xeem
| Channel | Kev txhais cov txiaj ntsig |
| FAM Channel | |
| Ct≤35 | E.coli O157:H7 Zoo |
| Undet | E.coli O157:H7 Negative |
| 35 | Kev tsis txaus ntseeg, rov ntsuas dua * |
*Yog hais tias qhov kev ntsuam xyuas ntawm FAM channel muaj tus nqi Ct ≤40 thiab qhia tau hais tias "S" zoo li qhov nthuav dav nkhaus, qhov tshwm sim tau txhais tias qhov zoo, txwv tsis pub nws yog qhov tsis zoo.
