中文Africa swine fever tus kab mob PCR kuaj cov khoom siv
Khoom npe
Africa swine fever tus kab mob PCR kuaj pom (Lyophilized)
Loj
48tests/kit, 50test/kitab
Npaj siv
Cov khoom siv no siv cov txheej txheem fluorescent PCR tiag tiag los kuaj xyuas DNA ntawmAfrica swine fever virus (ASFV)nyob rau hauv cov ntaub so ntswg kab mob xws li tonsils, lymph nodes thiab spleen thiab kua kab mob ntaub ntawv xws li tshuaj tiv thaiv thiab ntshav ntawm npua.Nws yog qhov tsim nyog rau kev tshawb nrhiav, kev kuaj mob thiab kev tshawb nrhiav kabmob kis ntawmAfrica swine fever tus kab mob.Cov khoom siv yog ALL-READY PCR SYSTEM (Lyophilized), uas muaj cov DNA amplification enzyme, cov tshuaj tiv thaiv tsis zoo, cov primers tshwj xeeb thiab cov kev sojntsuam xav tau rau kev kuaj pom fluorescent PCR.
Cov khoom txheem
| Cheebtsam | Pob | specification | Cov khoom xyaw |
| ASFV PCR Mix | 1 × lub raj mis (Lyophilized hmoov) | 50 Test | dNTPs, MgCl2, Primers, Probes, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 zoo-strip raj(Lyophilized) | 48 Test | ||
| Kev Tswj Zoo | 1 * 0.2ml tube (lyophilized) | 10 Kev Xeem | Plasmid muaj ASFV tshwj xeeb fragments |
| Kev daws teeb meem | 1.5 ml Cryotube | 500 ua | / |
| Kev tswj tsis zoo | 1.5 ml Cryotube | 200 ua | 0.9% NaCl |
Cia & Txee Lub neej
(1) Cov khoom siv tuaj yeem thauj tau ntawm chav tsev kub.
(2) Lub neej txee yog 18 lub hlis ntawm -20 ℃ thiab 12 lub hlis ntawm 2 ℃ ~ 30 ℃.
(3) Saib daim ntawv lo ntawm cov khoom siv rau hnub tsim khoom thiab hnub tas sijhawm.
(4) Cov lyophilized hmoov version reagent yuav tsum tau muab cia rau ntawm -20 ℃ tom qab dissolution thiab rov qab khov -thaw yuav tsum tsawg tshaj li 4 zaug.
Cov cuab yeej
GENECHECKER UF-150, UF-300 real-time fluorescence PCR ntsuas.
Daim duab ua haujlwm
a) Lub raj mis version:
b) 8 zoo-strip raj version:
Pcr Amplification
Pom zooKev teeb tsa
| Kauj ruam | Lub voj voog | Kub (℃) | Sijhawm | Fluorescence channel |
| 1 | 1 | 95 | 2 min | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s ib | Sau FAM fluorescence |
* Ceeb toom: Cov teeb liab ntawm FAM fluorescence channel yuav raug sau ntawm 60 ℃.
Txhais cov qhabnias xeem
| Channel | Kev txhais cov txiaj ntsig |
| FAM Channel | |
| Ct≤35 | ASFV Positive |
| Undet | ASFV Negative |
| 35 | Cov txiaj ntsig tsis txaus ntseeg, rov kuaj dua * |
*Yog hais tias qhov kev ntsuam xyuas ntawm FAM channel muaj tus nqi Ct ≤40 thiab qhia tau hais tias "S" zoo li qhov nthuav dav nkhaus, qhov tshwm sim tau txhais tias qhov zoo, txwv tsis pub nws yog qhov tsis zoo.
