中文Listeria monocytogenes PCR Detection Kit
Khoom npe
Listeria monocytogenes PCR Detection Kit
Loj
48tests/kit, 50test/kitab
Npaj siv
Listeria monocytogenes yog cov kab mob gram-zoo microbacterium uas tuaj yeem loj hlob ntawm 4 ℃ thiab 45 ℃.Nws yog ib qho ntawm cov kab mob tseem ceeb uas cuam tshuam rau tib neeg kev noj qab haus huv hauv cov zaub mov txias.Lub ntsiab tshwm sim ntawm tus kab mob yog septicemia, meningitis thiab mononucleosis.Cov khoom siv no tsim nyog rau kev kuaj pom zoo ntawm Listeria monocytogenes hauv cov khoom noj, cov qauv dej, cov quav, ntuav, cov kab mob-txhim kho cov kua thiab lwm yam qauv siv lub hauv paus ntsiab lus ntawm lub sijhawm fluorescence PCR.Cov khoom siv yog ALL-READY PCR SYSTEM (Lyophilized) , uas muaj cov DNA amplification enzyme, thim rov qab transcriptase, cov tshuaj tiv thaiv tsis zoo, cov primers tshwj xeeb thiab cov kev sojntsuam xav tau rau kev kuaj pom fluorescent PCR.
Cia & Txee Lub neej
(1) Cov khoom siv tuaj yeem thauj tau ntawm chav tsev kub.
(2) Lub neej txee yog 18 lub hlis ntawm -20 ℃ thiab 12 lub hlis ntawm 2 ℃ ~ 30 ℃.
(3) Saib daim ntawv lo ntawm cov khoom siv rau hnub tsim khoom thiab hnub tas sijhawm.
(4) Cov lyophilized hmoov version reagent yuav tsum tau muab cia rau ntawm -20 ℃ tom qab dissolution thiab rov qab khov -thaw yuav tsum tsawg tshaj li 4 zaug.
Cov khoom txheem
| Cheebtsam | Pob | specification | Cov khoom xyaw |
| Listeria monocytogenes PCR Mix | 1 × lub raj mis (Lyophilized hmoov) | 50 Test | dNTPs, MgCl2, Primers, Probes, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 zoo-strip raj(Lyophilized) | 48 Test | ||
| Kev Tswj Zoo | 1 * 0.2ml tube (lyophilized) | 10 Kev Xeem | Plasmid muaj Listeria monocytogenes tshwj xeeb fragments |
| Kev daws teeb meem | 1.5 ml Cryotube | 500 ua | / |
| Kev tswj tsis zoo | 1.5 ml Cryotube | 200 ua | 0.9% NaCl |
Cov cuab yeej
GENECHECKER UF-150, UF-300 real-time fluorescence PCR ntsuas.
Daim duab ua haujlwm
a)
b)
Pcr Amplification
Pom zooKev teeb tsa
| Kauj ruam | Lub voj voog | Kub (℃) | Sijhawm | Fluorescence channel |
| 1 | 1 | 50 | 8 feeb | |
| 2 | 1 | 95 | 2 min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s ib | Sau FAM fluorescence |
* Ceeb toom: Cov teeb liab ntawm FAM fluorescence channel yuav raug sau ntawm 60 ℃.
Txhais cov qhabnias xeem
| Channel | Kev txhais cov txiaj ntsig |
| FAM Channel | |
| Ct≤35 | Listeria monocytogenes zoo |
| Undet | Listeria monocytogenes tsis zoo |
| 35 | Kev tsis txaus ntseeg, rov ntsuas dua * |
*Yog hais tias qhov kev ntsuam xyuas ntawm FAM channel muaj tus nqi Ct ≤40 thiab qhia tau hais tias "S" zoo li qhov nthuav dav nkhaus, qhov tshwm sim tau txhais tias qhov zoo, txwv tsis pub nws yog qhov tsis zoo.
